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Characterization of Fish Serotonin, Dopamine and Norepinephrine Transporters as a Potential Target for Environmental Pharmaceuticals.

Created on 08 Sep 2026

Authors

Kikuko Honda, Minguang Han, Fuyuka Mori, Ayaka Morinaga, Yuka Nishimura, Renji Kaneko, Kie Oizumi, Hana Kajiyama, Mariko O Ihara, Han Zhang, Daisuke Kato, Kenji Toyota, Anke Lange, Charles R Tyler, Taisen Iguchi, Yuji Mushirobira, Masaki Nagae, Kiyoshi Soyano, Masaru Ihara, Shinichi Miyagawa

Published in

Environmental science & technology. Volume 60. Issue 35. Pages 24580-24590. Sep 08, 2026.

Abstract

Antidepressants are environmental contaminants widely prevalent in the environment that can affect aquatic organisms, but their underlying molecular mechanisms and species-specific sensitivities remain poorly understood. To address this knowledge gap, we performed a comprehensive molecular and pharmacological characterization of monoamine transporters (MATs), the primary targets of these pharmaceuticals, from two phylogenetically distinct teleost fish, medaka (Oryzias latipes) and ayu (Plecoglossus altivelis). We first cloned the cDNAs for two serotonin transporters (SERTa and SERTb), a dopamine transporter (DAT), and a norepinephrine transporter (NET) from both species. Pharmacological assays showed that fish SERTa is substantially more sensitive than SERTb to several antidepressants. While the overall inhibitory profiles of the antidepressants were generally conserved across species, fish SERTa orthologs were consistently and markedly more sensitive to human-targeted pharmaceuticals than the human SERT ortholog. Notably, inhibitory effects were also observed for several pharmaceuticals not traditionally recognized as targeting MATs in human. These molecular mechanisms findings help illustrate the vulnerability of aquatic wildlife to environmentally relevant concentrations of these pharmaceuticals.

PMID:
42708934
Bibliographic data and abstract were imported from PubMed on 08 Sep 2026.

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