Antibodies targeting a shared epitope exploit IgE allostery to drive distinct functional outcomes.

Authors

Anna M Davies, Nyssa Drinkwater, Andrew J Beavil, Victoria O'Dowd, Daniel Lightwood, Tom Ceska, Alistair J Henry, Brian J Sutton, James M McDonnell

Published in

Proceedings of the National Academy of Sciences of the United States of America. Volume 123. Issue 37. Pages e2613091123. Sep 15, 2026. Epub Sep 08, 2026.

Abstract

The Fc region of immunoglobulin E (IgE-Fc) is an important therapeutic target due to the antibody's critical role in allergic disorders through interactions with high-affinity (FcεRI) and low-affinity (CD23) receptors. IgE-Fc is known to be conformationally flexible, undergoing large-scale structural changes that modulate receptor binding and downstream biological functions. The mechanistic basis for how ligands induce these allosteric changes is not fully understood. In this study, we investigate structure-function relationships of IgE by characterizing four closely related anti-IgE-Fc Fab fragments with highly conserved sequences that recognize a shared epitope at the base of the Cε2 domain. Despite their sequence similarity, these Fabs exhibit markedly different binding characteristics, stoichiometries, and functional activities. Furthermore, the crystal structures of these four Fab complexes reveal that the bound IgE-Fc adopts a wide range of conformations. These structural differences directly dictate the functional outcomes, ranging from conformations that facilitate FcεRI binding to those that prevent or destabilize receptor interaction. Our findings demonstrate that highly similar antibodies binding to the same epitope on a protein that exhibits significant conformational plasticity can elicit distinct and divergent functional outcomes. This work challenges the conventional antibody discovery paradigm, i.e., "same epitope, same functional outcome," and highlights the importance of accounting for both the target's structural flexibility and the resulting functional diversity within antibody selection strategies. This is especially critical when targeting dynamic proteins where allosteric mechanisms are fundamental to function.

PMID:
42709784
Bibliographic data and abstract were imported from PubMed on 09 Sep 2026.

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