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scCGC2T: Curriculum-Guided Contrastive Co-Training Framework for scRNA-Seq Data Clustering.

Created on 09 Sep 2026

Authors

Zhenqiu Shu, Rong Dong, Tianyan Xu, Hongbin Wang, Zhengtao Yu

Published in

IEEE transactions on computational biology and bioinformatics. Volume PP. Sep 08, 2026. Epub Sep 08, 2026.

Abstract

Single-cell RNA sequencing (scRNA-seq) enables high-resolution analysis of cellular heterogeneity. Accurate scRNA-seq data clustering is crucial for cell type identification and subpopulation discovery. However, existing clustering methods are frequently affected by erroneous pseudo-labels generated through the static threshold, thus progressively accumulating errors during training. To address these challenges, in this paper, we propose a novel curriculum-guided contrastive co-training (scCGC${}^{2}$ T) framework for scRNA-seq data clustering. It designs a dual-branch architecture for cross-view contrastive co-training, thereby strengthening its representation ability for scRNA-seq data. Additionally, a progressive curriculum learning strategy is introduced to filter pseudo-labels dynamically. Specifically, high-confidence samples using the static threshold are applied to initial training, followed by guidance based on dynamic thresholding. Low-confidence samples are gradually incorporated into the training process, thereby mitigating the noise interference problem. Therefore, the proposed scCGC${}^{2}$ T method effectively mitigates error accumulation in the early training stage and greatly enhances the discriminative capability for complex cell subpopulations. Extensive experiments on several benchmarks demonstrate that the proposed method achieves superior clustering performance and strong generalization on scRNA-seq data clustering tasks. The source code for this work is available at: https://github.com/szq0816/scCGCT.

PMID:
42709526
Bibliographic data and abstract were imported from PubMed on 09 Sep 2026.

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