Protocol for fluorescence-activated mitochondria sorting in endoplasmic reticulum-mitochondria contact reporter cells.

Authors

Drew C Stark, Brandon Chen, Kayla Hickman, Costas A Lyssiotis, Yatrik M Shah

Published in

STAR protocols. Volume 7. Issue 3. Pages 104820. Sep 08, 2026. Epub Sep 08, 2026.

Abstract

Mitochondria exchange metabolites bidirectionally with the endoplasmic reticulum to maintain bioenergetic homeostasis and respond to cellular stress. Here, we describe a protocol for generating cells that contain a split-GFP contact-site reporter of ER-mitochondria interactions and the sorting of heterogeneous mitochondrial populations based on fluorescence intensity. This approach enables reproducible functional profiling of mitochondrial subpopulations, validated by proteomic analysis demonstrating the enrichment of endoplasmic reticulum-associated proteins in GFP-positive mitochondria. For complete details on the use and execution of this protocol, please refer to Chen et al.¹.

PMID:
42715106
Bibliographic data and abstract were imported from PubMed on 10 Sep 2026.

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