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Genetic Evidence Supports the Prioritization of CD40 Among Prespecified Immune-Related Candidate Drug Targets in Myasthenia Gravis.

Created on 12 Sep 2026

Authors

Yue Xu, Xiaolei Li, Guangxian Nan

Published in

The International journal of neuroscience. Pages 1-15. Sep 11, 2026. Epub Sep 11, 2026.

Abstract

To prioritize prespecified immune-related candidate drug targets in myasthenia gravis for further validation based on integrated genetic evidence.
We integrated drug-target Mendelian randomization (MR) using cis-expression quantitative trait loci (cis-eQTLs), protein-level MR of plasma CD40 abundance using plasma protein quantitative trait loci (pQTLs), and colocalization analyses to evaluate genetically proxied associations with overall MG, early-onset myasthenia gravis (EOMG), and late-onset myasthenia gravis (LOMG).
In this study, CD40 showed the most consistent genetic evidence among the six prespecified targets. Effect estimates are reported as odds ratios (ORs) with 95% confidence intervals (CIs). Higher CD40 expression proxied by cis-eQTLs was associated with increased risk of overall MG (OR = 1.14, 95% CI: 1.05-1.24, Bonferroni-adjusted P = 0.022) and EOMG (OR = 1.32, 95% CI: 1.12-1.56, Bonferroni-adjusted P = 0.015). Genetically predicted higher plasma CD40 protein abundance was associated with increased overall MG risk (OR = 1.31, 95% CI: 1.08-1.57, Bonferroni-adjusted P = 0.010), whereas the protein-level MR result for EOMG was directionally consistent but not statistically significant. Colocalization analysis provided suggestive but not definitive evidence of colocalization between CD40 expression and EOMG risk. FCGRT, IL2RA, and SYK showed additional exploratory MR signals requiring further validation.
CD40 showed the most consistent genetic support among the prespecified targets, supporting its prioritization for functional validation and further therapeutic investigation in MG.

PMID:
42727063
Bibliographic data and abstract were imported from PubMed on 12 Sep 2026.

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