Astrocytic Ca²⁺ activation by chemogenetics mitigates the effect of kainic acid-induced excitotoxicity on the hippocampus.

Authors

Nira Hernández-Martín, María Gómez Martínez, Pablo Bascuñana, Rubén Fernández de la Rosa, Luis García-García, Francisca Gómez, Maite Solas, Eduardo D Martín, Miguel A Pozo

Published in

Glia. Volume 72. Issue 12. Pages 2217-2230. Epub Aug 26, 2024.

Abstract

Astrocytes play a multifaceted role regulating brain glucose metabolism, ion homeostasis, neurotransmitters clearance, and water dynamics being essential in supporting synaptic function. Under different pathological conditions such as brain stroke, epilepsy, and neurodegenerative disorders, excitotoxicity plays a crucial role, however, the contribution of astrocytic activity in protecting neurons from excitotoxicity-induced damage is yet to be fully understood. In this work, we evaluated the effect of astrocytic activation by Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) on brain glucose metabolism in wild-type (WT) mice, and we investigated the effects of sustained astrocyte activation following an insult induced by intrahippocampal (iHPC) kainic acid (KA) injection using 2-deoxy-2-[¹⁸F]-fluoro-D-glucose (¹⁸F-FDG) positron emission tomography (PET) imaging, along with behavioral test, nuclear magnetic resonance (NMR) spectroscopy and histochemistry. Astrocytic Ca²⁺ activation increased the ¹⁸F-FDG uptake, but this effect was not found when the study was performed in knock out mice for type-2 inositol 1,4,5-trisphosphate receptor (Ip3r2^-/-) nor in floxed mice to abolish glucose transporter 1 (GLUT1) expression in hippocampal astrocytes (GLUT1^ΔGFAP). Sustained astrocyte activation after KA injection reversed the brain glucose hypometabolism, restored hippocampal function, prevented neuronal death, and increased hippocampal GABA levels. The findings of our study indicate that astrocytic GLUT1 function is crucial for regulating brain glucose metabolism. Astrocytic Ca²⁺ activation has been shown to promote adaptive changes that significantly contribute to mitigating the effects of KA-induced damage. This evidence suggests a protective role of activated astrocytes against KA-induced excitotoxicity.

PMID:
39188024
Bibliographic data and abstract were imported from PubMed on 12 Sep 2026.

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