Crosstalk between RNA secondary and three-dimensional structure prediction: a comprehensive study.

Authors

Deyin Wang, Yangwei Jiang, Linli He, Linxi Zhang, Ruhong Zhou, Dong Zhang

Published in

RNA biology. Volume 23. Issue 1. Pages 1-18. Dec 31, 2026. Epub Apr 13, 2026.

Abstract

In recent years, various computational methods have been developed to predict the three-dimensional (3D) structures of RNAs. Due to its hierarchical folding property, RNA secondary (2D) structure is often used as input for 3D structure prediction to improve accuracy and efficiency. However, the extent to which the accuracy of input 2D structure affects the performance of 3D structure prediction remains to be further investigated. Additionally, whether and how the input base-pairing interactions are modified during the 3D structure modelling process is another question worth exploring. To address these issues, here we comprehensively benchmark six representative 3D structure prediction models on extensive datasets, using 2D structures of varied accuracies as input. Our results indicate that there is a pervasive crosstalk between RNA 2D and 3D structure predictions, where the performance dependence of 3D structure prediction on the accuracy of input 2D structure is closely associated with the 3D model's ability to modify the input base-pairing interactions during structure modelling. Furthermore, we also observed that RNA 3D structure prediction performance is more sensitive to the occurrence of false positive base pairs in the input 2D structure than to true positive base pairs, suggesting a worthy direction to further improve the model performance.

PMID:
41923428
Bibliographic data and abstract were imported from PubMed on 12 Sep 2026.

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