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Functional and Structural Alterations Associated with Dehydration of Lactic Acid Bacteria.

Created on 14 Sep 2026

Authors

Nelson Romano, Monica Marro, Maria Marsal, Pablo Loza-Álvarez, Andrea Gomez-Zavaglia

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3060. Pages 723-734.

Abstract

The dehydration of lactic acid bacteria for technological purposes induces multilevel damage to bacterial cells. This chapter provides protocols for analyzing functional and structural alterations following the dehydration of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 using vacuum drying technology. Protocols involving conventional microbiological assays (plate counting, metabolic activity), fluorescence (membrane integrity), and Raman spectroscopies (DNA conformational analysis) are presented, along with experimental tips and suggestions.

PMID:
42732523
Bibliographic data and abstract were imported from PubMed on 14 Sep 2026.

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