Standardized Cryopreservation Protocols for European Perch (Perca fluviatilis) and Pikeperch (Sander lucioperca) Semen Enabling Hatchery-Scale Fertilization.

Authors

Sylwia Wałdowska, Joanna Nynca, Daniel Żarski, Katarzyna Palińska-Żarska, Mariola A Dietrich, Andrzej Ciereszko

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3060. Pages 339-349.

Abstract

Cryopreservation of fish semen ensures the year-round availability of gametes from species with seasonal spawning, supports genetic resource banking, and underpins selective breeding programs. However, percid species such as European perch and pikeperch pose specific challenges, notably the spontaneous activation of sperm motility in seminal plasma, which can reduce sperm motility reserves during cryopreservation. Building on systematic studies, here we present standardized protocols for semen collection, sperm quality evaluation, extender preparation, freezing, thawing, and post-thaw handling. These protocols support reliable commercial cryobanking and enable consistent, hatchery-scale fertilization in percid aquaculture.

PMID:
42732502
Bibliographic data and abstract were imported from PubMed on 14 Sep 2026.

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