High-Dose Aluminium Chloride Exposure Disrupts the Renal Cortical Injury-Repair Balance in Rats: Partial Modulation by L-Carnitine Pretreatment.

Authors

Faten S Abo-Zeid, Wiame W M Emam

Published in

Animals : an open access journal from MDPI. Volume 16. Issue 12. Jun 18, 2026. Epub Jun 18, 2026.

Abstract

Severe subacute exposure to aluminium chloride (AlCl₃) impairs renal function and induces cortical tubular injury; however, the concomitant balance between injury and repair in tubular epithelia remains incompletely defined. Accordingly, we aimed to use a high-dose regimen of AlCl₃ (100 mg·kg^-1·day^-1 for 30 days, oral gavage) as a standardised renal stressor in male Wistar rats to quantify shifts along the injury-repair balance in the renal cortex and to test whether L-carnitine (LC) pretreatment (200 mg·kg^-1·day^-1) can attenuate these shifts. Twenty rats were assigned to four groups: control, LC alone, AlCl₃ alone, and LC followed 60 min later by AlCl₃. On day 31, we assessed body-weight gain, renal functional markers, blinded cortical lesion scoring, quantitative histochemistry, and immunohistochemical profiling of cleaved caspase-3 (apoptotic signalling) and Ki-67 (proliferative engagement) within the same cortical compartment. AlCl₃ exposure produced a severe renal stress phenotype compared with controls, reducing body-weight gain from 99.8 ± 8.6 to 24.0 ± 8.3 g and increasing serum urea and creatinine from 26.40 ± 3.21 to 48.60 ± 5.81 mg/dL and from 0.606 ± 0.063 to 0.956 ± 0.147 mg/dL, respectively. Cortical injury increased from 0 (0-0) in controls to 15 (15-15) after AlCl₃ exposure. AlCl₃ also reduced strong PAS area from 97.92 ± 1.10% to 52.37 ± 14.68% and protein optical density from 0.353 ± 0.020 to 0.269 ± 0.039, while increasing collagen area fraction from 6.92 ± 1.67% to 18.40 ± 3.02% and cleaved caspase-3 from 1.0 (1.0-2.0) to 12.0 (12.0-12.0). Ki-67 labelling declined from 17.80 ± 3.35% to 6.00 ± 1.58%, indicating suppressed proliferative engagement. Compared with AlCl₃ alone, LC pretreatment showed partial protection, with higher body-weight gain (70.0 ± 15.6 g), lower serum urea and creatinine (21.40 ± 2.30 mg/dL and 0.580 ± 0.084 mg/dL), lower cortical injury burden [3 (3-4)], greater strong PAS area (89.25 ± 2.67%), higher protein optical density (0.354 ± 0.012), lower collagen area fraction (12.26 ± 1.70%), lower cleaved caspase-3 [4.0 (4.0-6.0)], and higher Ki-67 labelling (10.60 ± 2.30%). Residual cortical injury, persistent collagen elevation, and incomplete Ki-67 preservation indicate that LC pretreatment attenuated, but did not fully prevent, AlCl₃-induced renal cortical alterations. Overall, high-burden AlCl₃ exposure not only enhanced cell loss but also impaired regenerative renewal, whereas LC pretreatment partially preserved this injury-repair balance.

PMID:
42353505
Bibliographic data and abstract were imported from PubMed on 14 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.