Authors
Yanyan Wang, Peipei He, Tianle Chao, Qing Li, Lu Zhang, Jingsen Liang, Ying Wei, Jiaqing Hu, Jianmin Wang
Published in
Journal of animal science. Volume 103. Jan 04, 2025.
Abstract
This study performed transcriptome sequencing of ovarian tissues from JG goats at three key reproductive stages-non-pregnant (8 mo old, M8), pregnant (10 mo old, M10), and postpartum (12 mo old, M12). A total of 93 differentially expressed (DE) miRNAs were identified across all libraries, with the M8 vs. M10 group having the largest number (34 upregulated and 47 downregulated; |log₂ fold change| > 1, FDR < 0.05). These stage-specific DE miRNAs were clustered into four groups, with their target genes primarily enriched in signaling pathways such as Wnt, JAK-STAT, PI3K-Akt, VEGF, and steroid biosynthesis, which are important factors influencing ovarian cell activity, hormone secretion, angiogenesis, and embryonic development. Real-time quantitative polymerase chain reaction verification showed that the expression patterns of the 9 selected DE miRNAs were consistent with the sequencing data. Additionally, through the joint analysis of DE miRNAs and mRNAs, a co-expression network associated with ovarian reproductive function changes was constructed, ultimately highlighting 11 key miRNAs. Dual-luciferase reporter assays further confirmed the direct targeting relationships between core miR-432-5p and CPT1B, as well as miR-455-3p and PLXNB1. In summary, this study revealed the dynamic changes in ovarian miRNA expression profiles from pre-pregnancy to postpartum and their potential molecular mechanisms, providing theoretical support and key molecular targets for miRNAs in reproductive regulation, with significant implications for improving reproductive efficiency in livestock.
PMID:
40643221
Bibliographic data and abstract were imported from PubMed on 15 Sep 2026.
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