A haplotype-resolved chromosome-level genome assembly of autotetraploid Chinese yam (Dioscorea polystachya) elucidates dioscin biosynthesis and regulation.

Authors

Nan Shan, Yao Xiao, Tianyao Li, Putao Wang, Asjad Ali, Jingyu Sun, Shenglin Wang, Qianglong Zhu, Tianxu Cao, Sha Luo, Jiali Lin, Zihao Li, Qinghong Zhou, Yingjin Huang

Published in

Horticulture research. Volume 13. Issue 3. Pages uhaf344. Epub Dec 11, 2025.

Abstract

Chinese yam (Dioscorea polystachya) is extensively cultivated for nutritional and medicinal applications. However, the lack of a high-quality reference genome has hindered molecular genetic analysis and breeding advancements. Here, we present a haplotype-resolved chromosome-level assembly for this autotetraploid species, featuring a 1.56-Gb genome anchored to 80 chromosomes across four haplotypes and comprising 95 668 protein-coding genes. Following divergence from Dioscorea alata about 4.64 million years ago (Mya), D. polystachya underwent a specific whole-genome duplication ~1.42 Mya, resulting in an autotetraploid species without subgenomic dominance. Notably, the biosynthetic pathway genes of dioscin, an important steroidal saponin primarily accumulating in tubers, were generally over-retained in D. polystachya compared to the diploid species D. alata. Of these genes, 7-dehydrocholesterol reductase (Dp7-DR) promoted the accumulation of dioscin, exhibiting tuber-specific expression and strong inducibility by abscisic acid, based on transcriptome and gene function analyses. We determined that the transcription factor DpbZIP12 activates Dp7-DR transcription, as supported by yeast one-hybrid, dual-luciferase reporter, and electrophoretic mobility shift assays. Notably, overexpressing Dp7-DR or DpbZIP12 resulted in lower cholesterol levels and elevated dioscin levels, while silencing either gene produced opposite metabolic profiles. These findings delineate promising targets for manipulating dioscin content and expand genetic resources for enhancing yam nutritional quality.

PMID:
41821685
Bibliographic data and abstract were imported from PubMed on 15 Sep 2026.

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