Variable Temporal Interval OCTA Velocimetry Enables Wide-Range Hemodynamic Mapping in Hyperoxia Models.

Authors

Guocheng Xiao, Bin Ruan, Hefu Pan, Wanping Hu, Xuqiang Tian, Haitao Wang, Liqiang Wang

Published in

Investigative ophthalmology & visual science. Volume 67. Issue 11. Pages 26. Sep 01, 2026.

Abstract

The purpose of this study was to introduce variable interscan saturation alignment (VISA), an optical coherence tomography angiography (OCTA)-based method for wide-range flow velocimetry with angular tolerance, and validate its ability to quantify hyperoxia-induced hemodynamic changes in rat retina and cerebral cortex.
VISA achieved 92.6% mean velocity estimation accuracy over 1.0 to 20.0 mm/s, with a strong linear association between estimated and preset velocities (R² = 0.996), and stable measurements across 35 degrees to 90 degrees. In vivo imaging resolved layer-specific blood flow. Hyperoxia reduced flow velocity in the superficial vascular plexus, deep capillary plexus, and choroid by 21.88 ± 17.71%, 20.00 ± 10.91%, and 24.81 ± 15.18%, respectively, and superficial cortical flow velocity by 5.67 ± 1.81% (P < 0.05).
In phantom studies, VISA achieved a mean velocity estimation accuracy of 92.6% across the 1.0 to 20.0 mm/s range, with strong linear association between measured and preset velocities (R² = 0.996), and maintained stable measurements across the tested incident angles of 35 degrees to 90 degrees at 4 preset flow velocities (7.5, 10.0, 12.5, and 15.0 mm/s). In vivo imaging resolved layer-specific blood flow in the retina and cerebral cortex. Hyperoxia induced significant reductions in flow velocity in the superficial vascular plexus, deep capillary plexus, and choroid by 21.88 ± 17.71%, 20.00 ± 10.91%, and 24.81 ± 15.18%, respectively, and reduced superficial cortical flow velocity by 5.67 ± 1.81% (P < 0.05).
VISA enables wide-range quantitative OCTA velocimetry with angular tolerance under the tested phantom conditions and quantifies hyperoxia-induced microvascular responses in the rat retina and cerebral cortex, supporting volumetric flow mapping in retinal and cerebral microcirculation.

PMID:
42742215
Bibliographic data and abstract were imported from PubMed on 15 Sep 2026.

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