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Immune Toxicities in AAV Gene Therapy: Overview for Clinicians.

Created on 16 Sep 2026

Authors

Shestruma Parajuli, Thomas Gallagher, Terence R Flotte

Published in

International journal of molecular sciences. Volume 27. Issue 7. Mar 31, 2026. Epub Mar 31, 2026.

Abstract

Gene therapy using recombinant adeno-associated virus (rAAV) vectors has emerged as a transformative therapeutic modality for genetic disorders, demonstrating high transduction efficiency and a generally favorable safety profile during pre-clinical development. However, serious adverse events, including thrombotic microangiopathy, acute respiratory distress syndrome, hepatotoxicity, myocarditis, cytokine storm, and hemophagocytic lymphohistiocytosis, have been observed across multiple gene therapy clinical trials. Significant efforts have been made to understand the toxicities that cause these adverse events and clinical care for patients receiving gene therapies has evolved to mitigate their effects. These toxicities arise from a complex interplay between the innate and adaptive immune responses directed against the viral capsid and transgene products and are often compounded by pre-existing anti-AAV immunity. Immunomodulatory strategies have been developed to combat these responses to improve the long-term success of gene therapies, and this review provides clinicians managing gene therapy patients with an overview of mechanisms underlying AAV-associated immunotoxicities and a discussion of syndromes and mitigation strategies that have been reported in the clinical care of patients.

PMID:
41977378
Bibliographic data and abstract were imported from PubMed on 16 Sep 2026.

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