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Vitamin C Alleviates BPA Embryotoxicity by Attenuating Oxidative Stress and DNA Damage-Mediated Apoptosis via Modulation of the ATM/Chk2/p53 Pathway.

Created on 16 Sep 2026

Authors

Fei Ren, Zhen Lu, Xueping Cheng, Shitong Hao, Lingli Chen, Zhihong Yin, Dongfang Hu, Hongmei Ning, Yaming Ge, Junping He, Shanshan Shen, Wei Zhang

Published in

Journal of agricultural and food chemistry. Volume 74. Issue 14. Pages 11845-11857. Apr 15, 2026. Epub Mar 31, 2026.

Abstract

Bisphenol A (BPA), an environmental contaminant that migrates from food packaging, poses significant risks to embryonic development. This study investigated the protective effects and mechanisms of vitamin C (l-ascorbic acid) against BPA-induced embryotoxicity using Xenopus laevis embryos and P19 stem cells under a simultaneous exposure design. A multiassay approach was applied, including developmental bioassays, histological analysis, single-cell gel electrophoresis, and molecular profiling. The results showed that vitamin C significantly alleviated BPA-induced mortality, malformations, growth retardation, neurodevelopmental impairments, and notochord damage. Mechanistically, vitamin C restored the expression of antioxidant genes (gpx4, sod, ppard, and cat.2), reduced reactive oxygen species generation and oxidative DNA damage, and suppressed apoptosis via modulation of the ATM/Chk2/p53 signaling pathway. These findings demonstrate that vitamin C mitigates BPA-induced embryotoxicity by limiting oxidative stress and DNA damage-mediated apoptosis under coexposure conditions, supporting further evaluation of antioxidant-based protection across different exposure regimens and in combination with other antioxidants.

PMID:
41914611
Bibliographic data and abstract were imported from PubMed on 16 Sep 2026.

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