Endothelial and platelet dysfunction in systemic sclerosis with pulmonary hypertension.

Authors

Thamonwan Imerbtham, Thanaporn Sriwantana, Pintip Ngamjanyaporn, Thanyaluck Sotananusak, Pipat Ngammisri, Krittimeth Trerayapiwat, Panu Dumrongkitchaiporn, Prapaporn Pisitkun, Sirada Srihirun, Pornpun Vivithanaporn, Nathawut Sibmooh

Published in

PloS one. Volume 21. Issue 9. Pages e0357987. Epub Sep 16, 2026.

Abstract

Systemic sclerosis (SSc) is an autoimmune connective tissue disease characterized by vasculopathy, inflammation, and fibrosis, in which pulmonary hypertension (PH) is a major cause of mortality. In SSc, the relationship between endothelial and platelet functions with echocardiographic findings have not been evaluated. Here, we evaluated the endothelial and platelet functions in SSc patients with different PH probability and tested their associations with echocardiographic parameters, including the maximal tricuspid regurgitant velocity (TRVmax). Endothelial function was assessed by flow-mediated dilation (FMD) of brachial artery and blood nitrite levels, measured by chemiluminescence method. Platelet activity was measured by light transmission aggregometry in response to stimulation by adenosine diphosphate (ADP) and thrombin receptor-activating peptide-6 (TRAP-6). PH probability was assessed by echocardiography, and PH diagnosis was confirmed by right heart catheterization. SSc patients with PH probability had lower FMD than age-matched controls: FMD decreased from 13.3 ± 3.9% in controls to 5.6 ± 1.7%, 4.6 ± 0.4%, and 3.8 ± 0.4% in SSc patients with low PH probability, intermediate PH probability, and confirmed PH, respectively. SSc patients with PH had higher blood nitrite levels than controls or the patients with low PH probability. TRVmax correlated inversely with FMD and positively with platelet aggregation. In multivariable regression analysis, TRAP-6-induced platelet aggregation and FMD were independently associated with TRVmax. Furthermore, we demonstrated in two patients that nebulized sodium nitrite transiently reduced TRVmax. In conclusion, SSc patients have endothelial dysfunction and increased platelet activity, which exhibits correlation with TRVmax.

PMID:
42748184
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.

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