Authors
Negin Farzad, Archibald Enninful, Yao Lu, Fabio Parisi, Anthony Fung, Yumi Kwon, Yajuan Li, Margaux Labrosse, Mingyu Yang, Francesco Strino, Liang Chen, Junchen Yang, Mei Zhong, Fu Gao, Bo Tao, Joseph Cunningham, Zhiliang Bai, Haikuo Li, Fang Wang, Michael Stankewich, Dongjoo Kim, Mingze Dong, Lisa M Bramer, Keyi Li, Meera R Bhat, Evan Loe, Joseph Craft, Ljiljana Pasa-Tolic, Stephanie Halene, Lingyan Shi, Yuval Kluger, Mina L Xu, Rong Fan
Published in
Cell press blue. Volume 1. Issue 4. Jul 20, 2026.
Abstract
Immunosenescence is a hallmark of human aging and contributes to age-related immune decline, yet the development of senescence-associated phenotypes in the human lymphoid organs remains poorly understood. Here, we integrate single-cell and spatial multi-omics to systematically characterize age-related senescence in human lymph nodes (LNs) across the lifespan. Spatial proteomic profiling of 99 LN sections from 51 donors (18-86 years) using high-plex immunofluorescence (~20 million cells) mapped senescence markers (p16, p21, HMGB1, and γ-H2AX) at single-cell resolution, revealing diverse senescent-like cell types ("senotypes") and a stepwise shift from extrafollicular to germinal-center localization with age. In aged LNs, germinal-center B cells exhibit focal accumulation of senescence-associated programs, accompanied by impaired functional signatures, metabolic remodeling, and altered regulatory networks. These findings define a spatially organized landscape of immunosenescence in human lymphoid tissue and highlight germinal-center B cells as a key locus of age-associated immune dysfunction.
PMID:
42519544
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.
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