Qualitative and flow cytometric evaluations of goat's frozen semen in Mito-TEMPO supplemented plant-based extender.

Authors

Fatemeh Zarei, Nader Asadzadeh, Hassan Khamisabadi, Hoda Javaheri Barfourooshi, Ali Hatefi, Reza Masoudi, Mohsen Sharafi

Published in

BMC veterinary research. Volume 22. Issue 1. Apr 30, 2026. Epub Apr 30, 2026.

Abstract

Cryopreservation of buck spermatozoa is an efficient strategy to improve qualified sperm cells for artificial insemination; however, this process potentially decreases the qualitative characteristics of post-thawed sperm. Therefore, this research aimed to evaluate the influence of the mitochondria-targeted antioxidant "Mito-TEMPO" on buck sperm quality during the cryopreservation process in a plant-based extender.
This study indicated that the dosages 10 and 100 µM Mito-TEMPO presented higher (P ≤ 0.05) percentages of total motility, progressive motility, membrane integrity, mitochondrial activity, acrosome integrity, and viability while showing lower (P ≤ 0.05) late apoptosis, DNA fragmentation, reactive oxygen species (ROS) concentration, and lipid peroxidation compared to the control group. Different treatments of Mito-TEMPO indicated no effect (P > 0.05) on sperm cells with abnormal morphology.
It can be concluded that this mitochondria-targeted antioxidant may be able to preserve the qualitative parameters in the post-thawed buck spermatozoa.

PMID:
42062996
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.

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