Cdc42 Activation in Antinephrin Antibody-Induced Nephropathy.

Authors

Ying Zhang, Yoshiyasu Fukusumi, Hidenori Yasuda, Guoqing Chang, Mutsumi Kayaba, Hiroshi Kawachi

Published in

Journal of the American Society of Nephrology : JASN. Volume 36. Issue 11. Pages 2164-2176. Nov 01, 2025. Epub Jun 03, 2025.

Abstract

Antinephrin antibodies phosphorylated nephrin and ephrin-B1, and phosphorylated ephrin-B1 released Par6 and promoted cdc42 activity. Elevated cdc42 activity decreased nephrin and ephrin-B1 and induced claudin1 expression by regulating Snail and Stat3. The regulation of cdc42 activity might be a promising therapy for minimal change nephrotic syndrome induced by autoantibodies against nephrin.
A subset of minimal change nephrotic syndrome was reported to be induced by autoantibodies against nephrin. We have reported that rats injected with murine antinephrin antibody showed proteinuria. However, its precise pathogenic mechanisms remain unclear.
The initiation events of podocyte disturbance caused by antinephrin antibodies were analyzed using an in vivo rat model and in vitro assays with rat isolated glomeruli and human cultured podocytes. To elucidate the role of ephrin-B1 at the slit diaphragm, podocyte-specific ephrin-B1 knockout mice were analyzed.
Nephrin-binding ephrin-B1 at slit diaphragm interacted with Par6 and interfered with the binding of Par6 with cdc42. Antinephrin antibodies caused the phosphorylations of nephrin and ephrin-B1 in a TRPC6-mediated Ca²⁺ influx–dependent manner. Phosphorylated ephrin-B1 was dissociated from nephrin and also from Par6. Par6, released by ephrin-B1, interacted with cdc42. The binding of Par6 stabilized cdc42 and consequently promoted cdc42 activity. Elevated cdc42 activity increased calcineurin activity and consequently activated Snail. The activated Snail negatively regulated the mRNA expressions of the slit diaphragm functional molecules, nephrin and ephrin-B1. Elevated cdc42 activity activated Stat3 independently of calcineurin. The activated Stat3 brought on the expression of claudin1, a tight junction molecule. The altered expressions of these molecules at the protein level were observed in the rat antinephrin antibody–induced nephropathy when the rats showed proteinuria.
Ephrin-B1 at slit diaphragm suppresses cdc42 activity by preventing the interaction of Par6 with cdc42 and functions to keep the specialized phenotype of podocytes. Elevated cdc42 activity induced by the binding of Par6, released by the phosphorylated ephrin-B1, is a critical initiation event leading to proteinuria in the antinephrin antibody–induced nephropathy.

PMID:
40459930
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.