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GWAS identifies regulators of seed and leaf morphology in common bean, revealing OFP5 as a major seed size determinant.

Created on 17 Sep 2026

Authors

Julia von Steimker, Mustafa Bulut, Gregory Pozhvanov, Susan Bergmann, Regina Wendenburg, Yuming Sun, Feng Zhu, Alice Pieri, Giulia Frascarelli, Laura Nanni, Elisa Bellucci, Elena Bitocchi, Roberto Papa, Alisdair R Fernie, Saleh Alseekh

Published in

The Plant journal : for cell and molecular biology. Volume 126. Issue 3. Pages e70909.

Abstract

Phaseolus vulgaris (common bean) is one of the most economically important members of the Fabaceae family and a crop of high nutritional value. Seed and leaf morphological traits are key determinants of yield, shaped by genetic and environmental factors. In this study, we characterized a diversity panel of 434 P. vulgaris accessions for morphological and colorimetric traits. Using genome-wide association studies based on both single-nucleotide polymorphisms and structural variants, we identified 73 high-confidence quantitative trait locus (QTL). In total, we present 114 candidate genes within 25 quantitative trait loci linked to seed and leaf morphology and color, including an OVATE Family Protein 5 (PvOFP5), which was functionally validated as a key regulator of seed size in common bean. We employed a heterologous approach by overexpressing the inferior (Mesoamerican) and superior (Andean) alleles of PvOFP5 in Arabidopsis thaliana wild-type and knockout lines, and confirmed the key role of PvOFP5 in determining seed area. This work provides a comprehensive atlas of genetic associations for bean morphology and color, and highlights PvOFP5 as a promising target for marker-assisted breeding aimed at optimizing seed size.

PMID:
42089377
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.

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