Authors
Nena Hilmia, Indrawati Yudha Asmara, Dani Garnida, Muhammad Ihsan Andi Dagong, Sri Rachma Aprilita Bugiwati, Sutopo Sutopo, Dela Ayu Lestari, Asep Setiaji, Fajrin Shidiq
Published in
Journal, genetic engineering & biotechnology. Volume 24. Issue 3. Pages 100730. Epub Jun 23, 2026.
Abstract
Native chickens are essential genetic resources in Indonesia, providing economic, cultural, and nutritional value with strong adaptability to local environments. Among these native chickens, Sentul and Pelung are recognized as national genetic resources due to dual-purpose and ornamental traits, respectively, but their whole mitogenome characterization remains limited. Therefore, this study aimed to assemble and analyze the whole mitogenome of Sentul and Pelung chickens as well as perform a comparison with 35 additional genomes from domestic chickens and jungle fowls across Asia. The experiment was carried out using next-generation sequencing and bioinformatics-based genome assembly and analysis. The results showed that both Sentul and Pelung genomes were 16,784 bp in length and contained the typical mitochondrial gene composition, including 13 protein-coding genes (PCGs), 22 transfer ribonucleic acids (tRNAs), two ribosomal ribonucleic acids (rRNAs), and a control region (D-loop). Furthermore, comparative analysis identified five single-nucleotide polymorphisms (SNPs) distinguishing the two breeds, located in ND1, COX1, COX2, ND4, and the D-loop region. Phylogenetic reconstruction based on whole mitochondrial sequences showed that Sentul and Pelung chickens belong to haplogroup D, alongside other native breeds and red jungle fowls from Indonesia and the Philippines.
PMID:
42749411
Bibliographic data and abstract were imported from PubMed on 17 Sep 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 7
- Comments 0