An automated high-resolution screening platform identifies regulators of anchor cell invasion in C. elegans.

Authors

Simon Berger, Silvan Spiri, Evelyn Lattmann, Stefanie Engleitner, Mitchell P Levesque, Andrew deMello, Alex Hajnal

Published in

Science advances. Volume 12. Issue 38. Pages eaef6546. Sep 18, 2026. Epub Sep 18, 2026.

Abstract

Microfluidic devices are valuable tools for live imaging. However, widespread adoption of microfluidic-based screening methods has been limited by the complexity of the existing techniques. Here, we introduce a user-friendly, high-throughput, and high-resolution automated imaging system for C. elegans. We demonstrate the system's capabilities in an RNA interference (RNAi) screen, combined with neural network-based phenotypic scoring. We evaluated the effects of RNAi targeting 193 candidate genes on anchor cell (AC) invasion, a model for basement membrane (BM) breaching that shares similarities with tumor cell invasion during cancer metastasis. Over 40,000 animals were imaged at subcellular resolution and scored using a custom neural network classifier with an accuracy of over 92%. The screen identified 41 of 52 genes previously known to control AC invasion, along with 51 additional regulators of invasion. This automated imaging and classification system enables researchers to perform forward mutagenesis, RNAi, and drug screens in C. elegans with much greater speed and higher resolution than previously possible.

PMID:
42758833
Bibliographic data and abstract were imported from PubMed on 19 Sep 2026.

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