Protocol for identifying cellular and molecular signatures from spatial profiling data using micro-tissue analysis.

Authors

Huiqian Hu, Alphonsus H C Ng, Yue Lu

Published in

STAR protocols. Volume 7. Issue 4. Pages 104825. Sep 18, 2026. Epub Sep 18, 2026.

Abstract

Spatial tissue profiling measures hundreds to thousands of biomolecules across tissue regions, but most analyses examine each feature one at a time. Here, we present a protocol for micro-tissue analysis using partial least-squares modeling. We describe steps for stratifying regions by microenvironment, building multivariate models to identify which features collectively drive a biological outcome, and interpreting the results. We detail four worked examples using two published GeoMx digital spatial profiler (DSP) datasets from human tumor and mucosal tissues. For complete details on the use and execution of this protocol, please refer to Lu et al.¹ and Ng et al.².

PMID:
42758588
Bibliographic data and abstract were imported from PubMed on 19 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.