Ultrasensitive fluorescent biosensor for miRNA-34a detection: Magnetic separation coupled with cascade amplification of duplex-specific nuclease-assisted and hybridization chain reaction.

Authors

Yanjie Zheng, Xinyi Zhang, Siyi Wang, Kun Yang, Wanrong Chen, Chunyan Liu, Wei Chen, Mengxiang Su

Published in

Nanomedicine : nanotechnology, biology, and medicine. Pages 103027. Sep 18, 2026. Epub Sep 18, 2026.

Abstract

Lung cancer is the most common and lethal malignancy worldwide, with non-small cell lung cancer (NSCLC) accounting for approximately 85% of cases and exhibiting high mortality rates. MicroRNA-34a (miRNA-34a) is a promising biomarker, therapeutic target, and prognostic indicator for NSCLC, necessitating sensitive and specific detection methods for early diagnosis and improved clinical management. Here, we developed a highly sensitive fluorescent biosensor for miRNA-34a detection by integrating magnetic separation with cascade amplification of duplex-specific nuclease (DSN) and hybridization chain reaction (HCR). Under optimal conditions, the biosensor exhibits a wide linear range (1.0 fM-10.0 nM) and an ultralow detection limit (0.3 fM), with excellent specificity for discriminating against interfering miRNAs. Moreover, the sensor was successfully validated in clinical blood samples, showing excellent agreement with RT-qPCR results. Collectively, this method provides a sensitive and specific tool for trace miRNA-34a detection, demonstrating significant potential for early NSCLC diagnosis and improved patient management.

PMID:
42759861
Bibliographic data and abstract were imported from PubMed on 19 Sep 2026.

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