Authors
Riham Ayoubi, Sara González Bolívar, Richard A Kahn, Vincent Francis, Peter S McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group and ABIF consortium
Published in
F1000Research. Volume 15. Pages 1129. Epub Jul 10, 2026.
Abstract
Netrin-1 is a secreted protein that regulates cell migration and survival, controlling axonal guidance during development, morphogenesis, apoptosis, angiogenesis, inflammation and cancer progression. Here we have characterized fourteen Netrin-1 commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
PMID:
42760910
Bibliographic data and abstract were imported from PubMed on 19 Sep 2026.
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