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Mesoporous medium-entropy nanozyme with enhanced peroxidase-like activity: design, mechanism, and application as a sensor for ultrasensitive detection of trace thiophanate methyl.

Created on 20 Sep 2026

Authors

Yuhao Liao, Jianwen Tian, Minghui Luo, Wenting Zhu, Hailong Peng

Published in

Analytica chimica acta. Volume 1422. Pages 346076. Nov 08, 2026. Epub Aug 05, 2026.

Abstract

Medium-entropy alloy nanozyme (MEAzyme) with mesoporous framework, unique regular morphology, and excellent enzyme-like catalytic performance have shown great application potential. However, synthesizing MEAzymes that balance well-defined mesoporous structures and preserve unique regular morphology remains a challenge. Herein, in this study, a universal solvent-mediated polymerization-induced self-assembly (PISA) strategy was successfully used to design a mesoporous FeCuMn-MEAzyme with a regular and uniform spherical morphology.
The mesoporous FeCuMn-MEAzyme exhibits superior POD-like catalytic performance, which can be attributed to the "cocktail effect" of multimetallic active centers, high specific surface area (up to 236.40 m2/g), and large pore size (2.85∼13.09 nm). Interestingly, the POD-like activity of this nanozyme can be immediately and strongly by the thiophanate-methyl (TM). Accordingly, a FeCuMn-MEAzyme colorimetric sensor was developed for the ultrasensitive (LOD = 0.83 ng/mL) and selective detection of TM with showing an outstanding linear relationship over the range of 0.0010 μg/mL to 40.00 μg/mL. In addition, a rapid smartphone-integrated FeCuMn-MEAzyme colorimetric sensing platform was also constructed, which achieved a low LOD (0.78 ng/mL) for TM detection.
The developed FeCuMn-MEAzyme-based method was successfully applied to detect TM in environmental samples. This work provides a universal synthesis strategy for mesoporous MEAzyme with regular morphology and enhanced POD-like activity, demonstrating great potential in analytical and sensor applications.

PMID:
42763144
Bibliographic data and abstract were imported from PubMed on 20 Sep 2026.

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