TRPML1 loss disrupts lysosome-mitochondria homeostasis and sensitizes MDA-MB-231 triple-negative breast cancer cells to chemotherapy.

Authors

Alia K Syeda, Shekoufeh Almasi, Cory J Benson, Barry E Kennedy, Ryan M Yoast, Scott M Emrich, Amari Delima-Baril, Logan Slade, Shanmugasundaram Pakkiriswami, Vishnu V Vijayan, Unnikrishnan B Sheela, Shashi Gujar, Thomas Pulinilkunnil, Mohamed Trebak, Yassine El Hiani

Published in

The Journal of biological chemistry. Pages 113596. Sep 22, 2026. Epub Sep 22, 2026.

Abstract

Signaling mechanisms at the lysosome-mitochondria interface form a critical network that enables cancer cells to maintain mitochondrial quality control, adapt to metabolic stress, and survive therapy. However, the incomplete understanding of the mechanisms coordinating this network has limited the development of effective therapies, especially for triple-negative breast cancers (TNBC). Here, we identify TRPML1 as an important regulator of lysosome-mitochondrial communication in MDA-MB-231 TNBC cells. We find that TRPML1 knockdown (ML1-KD) impaired mitochondrial respiration, oxidative substrate utilization, ATP production and redox balance in MDA-MB-231 cells, whereas comparable changes were not observed in non-cancerous MCF10A cells. ML1-KD reduced lysosomal acidification and impaired autophagic flux and was accompanied by reduced TFEB nuclear localization, impaired mitophagy, and alterations in mitochondrial maintenance proteins. These changes were accompanied by organellar proximity remodelling, with increased mitochondria-ER proximity and reduced mitochondria-lysosome proximity, together with altered cytosolic/mitochondrial Ca²⁺ responses, broad metabolic remodelling, G0/G1 arrest, and caspase-3/7-independent cell death. Importantly, ML1-KD cells showed enhanced responses to otherwise subeffective concentrations of doxorubicin and paclitaxel. Together, our findings support TRPML1-dependent lysosomal signaling as an important contributor to mitochondrial-metabolic resilience and chemotherapy responsiveness in MDA-MB-231 TNBC cells.

PMID:
42772563
Bibliographic data and abstract were imported from PubMed on 23 Sep 2026.

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