Household LED Light-Mediated Photodynamic Therapy for Effective Inhibition of Multidrug-Resistant Staphylococcus aureus.

Authors

Lama Misba, Farheen Akhtar, Sameera Mujahid, Asad U Khan

Published in

Journal of biophotonics. Volume 19. Issue 9. Pages e70358.

Abstract

This study evaluates toluidine blue-O and curcumin (CUR) as photosensitizers (PSs) for photodynamic therapy against Staphylococcus aureus, each activated using a household light-emitting diode bulb selected to approximate the absorption profiles of the respective PS. The colony-forming unit (CFU) assay revealed time-dependent bacterial reductions, achieving 4.85 log₁₀ CFU/mL at 10 min for TBO and 4.26 log₁₀ CFU/mL at 15 min for CUR under their respective LED illumination conditions. Extracellular polymeric substance production decreased by 96.22% with TBO and 48.98% with CUR, accompanied by enhanced reactive oxygen species generation. Confocal laser scanning microscopy provided semi-quantitative evidence of an increased dead-to-live cell ratio following PDT. In vivo results, supported by histopathological examination and cytokine expression profiling, demonstrated modest but significant bacterial load reduction and improved tissue architecture following TBO- and CUR-mediated PDT. These findings suggest that readily available household LED bulbs may serve as accessible illumination sources for aPDT against S. aureus.

PMID:
42773775
Bibliographic data and abstract were imported from PubMed on 23 Sep 2026.

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