Authors
Wanjing Liu, Haozhe Zhou, Xiaogang Wang, Yuhang Chen, Jingjie Ma, Dejing Yin, Jianying Qian, Xiaomei Zhang, Guoqiang Xu, Jinsong Shi, Zhenghong Xu
Published in
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. Volume 42. Issue 9. Pages 4106-4123. Sep 25, 2026.
Abstract
Poly-γ-glutamic acid (γ-PGA) is a biopolymer polymerized from l-glutamic acid (l-Glu) and/or d-glutamic acid monomers, exhibiting broad application prospects in pharmaceuticals, cosmetics, and other fields. The low yields remain a key factor limiting large-scale production of γ-PGA in heterologous expression systems. To enhance the biosynthetic efficiency, this study employed Corynebacterium glutamicum as the chassis cell and utilized the γ-PGA synthase PgsBCA from Bacillus licheniformis to catalyze γ-PGA synthesis. First, a polycistron expression system was constructed, increasing the γ-PGA yield by 20.6% compared with the monocistron system. After site-directed and saturation mutagenesis of PgsA, the mutant K76C was identified, increasing the γ-PGA yield by 75.3%. On this basis, a Bayesian model was employed to optimize seven medium components including glucose and urea. The optimized medium achieved a γ-PGA yield of 14.52 g/L, which represented a 28.6% increase over that in the initial medium. Finally, the recombinant strain was scaled up in a 5 L fermenter. After 48 hours of fermentation, the γ-PGA yield reached 64.13 g/L, with a glucose conversion rate of 0.52 g/g and the l-Glu content of 100%. This study developed a high-yielding γ-PGA-producing engineered strain and established an efficient fermentation process, providing a novel strategy for the efficient production of γ-PGA.
PMID:
42773664
Bibliographic data and abstract were imported from PubMed on 23 Sep 2026.
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