Protocol for measuring fatty acid absorption and intracellular lipid accumulation in intestinal cells and organoids.

Authors

Jiaqi Wu, Yu Gu, Shuangshuang Liu, Shupei Wang, Li-Hao Huang

Published in

STAR protocols. Volume 7. Issue 4. Pages 104853. Sep 23, 2026. Epub Sep 23, 2026.

Abstract

Quantitative assessment of intestinal epithelial cells is essential for studies of metabolism and disease. Here, we present a protocol to quantify fatty acid uptake and lipid accumulation in intestinal epithelial cell lines and primary intestinal organoids using a flow cytometry-based approach with BODIPY FL C₁₆. We describe steps for assessing fatty acid uptake and accumulation in intestinal epithelial cell lines and intestinal organoids. This rapid and reproducible technique can also be adapted to investigate fatty acid metabolism in other cell types. For complete details on the use and execution of this protocol, please refer to Wu et al.¹.

PMID:
42776837
Bibliographic data and abstract were imported from PubMed on 24 Sep 2026.

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