Authors
Zhoujie Ma, Yongchuan Gan, Qiyun Liang, Lufei Yuan, Lele Song, Rong Jiang, Jilu Shen
Published in
Frontiers in microbiology. Volume 17. Pages 1934648. Epub Sep 09, 2026.
Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV), a novel bunyavirus associated with febrile illness, thrombocytopenia, and leukopenia, represents a considerable public health threat.
In this study, we developed a visual detection platform for SFTSV by integrating reverse transcription-recombinase polymerase amplification (RT-RPA) with lateral flow nucleic acid chromatography assay (LFNA). The method operates under isothermal conditions, and delivers results within 40 minutes. Using a conserved region of the S segment as the amplification target.
The assay demonstrated a detection limit of 101 copies/μL and showed no cross-reactivity with 12 other common clinical pathogens or 6 less common clinical pathogens. An evaluation of 80 SFTSV-positive clinical samples and 16 SFTSV-negative clinical samples showed that, compared with quantitative PCR (qPCR), the method demonstrated excellent sensitivity and specificity.
The RT-RPA-LFNA platform is easy to operate, provides rapid results and is cost-effective, and therefore has potential for use in point-of-care testing (POCT), particularly in resource-limited primary healthcare settings. This approach not only provides a reliable tool for SFTSV diagnosis but also holds potential for the detection of other pathogens.
PMID:
42780294
Bibliographic data and abstract were imported from PubMed on 24 Sep 2026.
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