Transposon engineering of Saccharomyces cerevisiae for allantoin-inducible cordycepin production.

Authors

Chaoqun Tang, Junyi Wang, Xueyi Luo, Haofeng Chen, Yang Zhang, Jifeng Yuan

Published in

Engineering in life sciences. Volume 26. Issue 8. Pages e009. Epub Aug 24, 2026.

Abstract

Cordycepin is a high-value bioactive compound with significant therapeutic potential. In this study, we report a transposon-based genome engineering strategy for establishing an allantoin-inducible cordycepin production platform in Saccharomyces cerevisiae. We first optimized the allantoin-inducible system by refining the culture medium's components to improve cell growth and achieved an approximately 50-fold on/off ratio after reaching a plateau, thereby enhancing the robustness of engineered yeast strains. Next, a multiselection marker transposon system was implemented to achieve combinatorial integration of cordycepin biosynthetic genes (cns1/cns2) into the yeast genome. Engineered yeast strains obtained from the combinatorial library displayed varying cordycepin titers. Among them, one engineered strain produced up to 135 mg/L cordycepin, which is significantly higher than that achieved by the episomal plasmid-based strain. This work demonstrated a transposon-based workflow for combinatorial pathway optimization, which will be of particular interest for future microbial cell factory design.

PMID:
42781467
Bibliographic data and abstract were imported from PubMed on 25 Sep 2026.

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