Authors
Matheus Zavadinack, Ester Mazepa, Rafael F N Quadrado, Zhenghao Zhai, Shayane da Silva Milhorini, Dib Mady Diniz Gomes, Glaucia R Martinez, Lucimara M C Cordeiro, Kevin J Edgar, Marcello Iacomini
Published in
Carbohydrate polymers. Volume 391. Pages 125797. Nov 01, 2026. Epub Aug 26, 2026.
Abstract
Mushroom-derived (1→3),(1→6)-β-D-glucans exhibit diverse biological activities, including effects relevant to cancer research, which may be modulated through chemical modification. In the present study, sequential hydroxypropylations were performed on a highly purified (1→3),(1→6)-β-D-glucan (NM-Glc) from the mushroom Lactarius quieticolor to generate derivatives with distinct structural characteristics (HP1 and HP2). The derivatives were characterized by NMR, GC-MS, and SEC-MALS-RI, which suggested preferential hydroxypropyl substitution at the O-6 and O-4 positions. Each sample (NM-Glc (unmodified glucan; Mw 1.26 × 105 g.mol-1), HP1 (Mw 4.02 × 104 g.mol-1, MS: 0.70, DSFrac: 0.38, DSSub: 0.57), and HP2 (Mw 5.66 × 104 g.mol-1, MS: 0.94, DSFrac: 0.47, DSSub: 0.83) was evaluated for its biological effects on B16-F10 murine melanoma cells at 10, 100, and 1000 μg.mL-1. NM-Glc did not exhibit significant cytotoxicity toward tumor cells under the evaluated conditions, whereas the hydroxypropyl-modified glucans exhibited cytotoxicity at 100 and 1000 μg.mL-1 and reduced cell adhesion to the substrate (observed only for HP2 at 1000 μg.mL-1). Acridine orange/ethidium bromide (AO/EB) staining revealed an increased proportion of apoptotic-like cells following treatment with HP1 and HP2, with HP2 producing the most pronounced effects. Therefore, hydroxypropylation is a promising strategy to generate (1→3),(1→6)-β-D-glucans capable of reducing melanoma cell viability in vitro.
PMID:
42785847
Bibliographic data and abstract were imported from PubMed on 25 Sep 2026.
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