Authors
Vera Ruíz Moleón, Sara González Bolívar Bolívar, Riham Ayoubi, Vincent Francis, Peter S McPherson, Carl Laflamme
Published in
F1000Research. Volume 15. Pages 1065. Epub Jul 04, 2026.
Abstract
Prosaposin functions as a regulator of lysosomal enzymatic activity and as a neuroprotective player when secreted. Here we have characterized nine Prosaposin commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
PMID:
42802730
Bibliographic data and abstract were imported from PubMed on 28 Sep 2026.
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