Authors
Suruchi Poddar, Dorothea A Erxleben, Rebecca J Dodd, Heidi L Reesink, Dixy E Green, Paul L DeAngelis, Anthony J Day, Adam R Hall
Published in
Proceedings of the National Academy of Sciences of the United States of America. Volume 123. Issue 40. Pages e2611716123. Oct 06, 2026. Epub Sep 28, 2026.
Abstract
The glycosaminoglycan hyaluronan (HA) is an essential and ubiquitous component of human tissues and biofluids. The only known covalent modification of HA entails the attachment of heavy chains (HC) from the inter-alpha-inhibitor (IαI) family of proteoglycans, forming stable complexes (HC·HA) that arise during inflammation. In some contexts, HC·HA is thought to contribute to pathology, whereas in others it may form part of a protective pathway. However, its exact roles are not fully understood. Here, we report that HC modifications can protect HA from fragmentation by reactive oxygen species (ROS) produced during the inflammatory cascade. Using solid-state nanopore molecular size analysis, we show that HA is highly resistant to degradation from exogenous ROS in vitro when in the context of HC·HA complexes, while the unmodified HA polymer is fragmented rapidly under the same conditions. Experiments performed with admixtures of HA and unbound antioxidant proteins-including HC-bearing components-demonstrate that covalent HC attachment to the polysaccharide is necessary for the protection. Finally, we find that HA with high-HC content from "inflammatory" equine synovial fluid has increased resilience to ROS damage compared to low-HC·HA from a healthy joint. Collectively, these results demonstrate that covalent HC modification is an effective biological strategy for preserving HA integrity against ROS fragmentation, including in inflammatory conditions.
PMID:
42804629
Bibliographic data and abstract were imported from PubMed on 29 Sep 2026.
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