Heterotrimeric G proteins containing Gβ1γ2 subunits exhibit subtype-specific mobility differences in live cells.

Authors

Ondřej Kuchynka, Andrei Kovalchuk, Mia Nußbaumer, Ekaterina Sviridova, Tomáš Fessl, Alexey Bondar

Published in

PloS one. Volume 21. Issue 9. Pages e0359304. Epub Sep 29, 2026.

Abstract

Heterotrimeric G proteins are key signal transducers in all eukaryotic cells. Anchored to the inner leaflet of the plasma membrane, they transmit and amplify extracellular chemical and physical signals downstream of G protein-coupled receptors. Despite numerous available studies, many biophysical aspects that regulate G protein signaling, including membrane mobility, remain poorly understood. Here, using single-molecule imaging, we show that different subtypes of heterotrimeric G proteins containing Gβ1γ2 subunits exhibit high diversity in their membrane mobility. We demonstrate that the nature of the Gα subunit plays a major role in defining the mobility of such heterotrimers. Our results indicate that heterotrimers containing Gα12 and Gα13 subunits exhibit markedly reduced mobility compared to those containing Gαi/o and Gαs subunits. These findings identify subtype-specific lateral membrane mobility of G proteins as a factor that can affect their signaling dynamics in living cells.

PMID:
42809568
Bibliographic data and abstract were imported from PubMed on 30 Sep 2026.

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