Protocol for quantification of AAV-CRISPR-mediated hepatic gene editing in mice.

Authors

Kelsey E Jarrett, Rodrigo Baltazar-Nunez, Chloe Anne Borja, Precious Juvie Calderon, Rochelle W Lai, Angela Cheng, Manasvi Paudel, Gabriella E Rubert, Anthony Suberski, Owen K Traina, Sunny Y Cai, Kalyani Srikanth, William R Lagor, Elizabeth J Tarling, Thomas Q de Aguiar Vallim

Published in

STAR protocols. Volume 7. Issue 4. Pages 104849. Sep 29, 2026. Epub Sep 29, 2026.

Abstract

Adeno associated viral (AAV) delivery of CRISPR-Cas9 enables efficient, targeted hepatic gene disruption composed of both small insertions and deletions (INDELs) and larger DNA structural variants (DNA-SVs), which are difficult to quantify. Here, we present a protocol for quantifying AAV-CRISPR-mediated gene editing in mice. We describe guide RNA (gRNA) design, plasmid cloning, delivery of AAV-CRISPR to the liver, and execution of liver DNA-editing analysis. This protocol enables quantification of INDELs and DNA-SVs, allowing for validation of gene disruption. For complete details on the use and execution of this protocol, please refer to Chan et al.¹.

PMID:
42809437
Bibliographic data and abstract were imported from PubMed on 30 Sep 2026.

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