Flow Cytometric Immunophenotyping of Acute Lymphoblastic Leukemia.

Authors

Dana Delgado, Jolene L Cardinali, Joseph A DiGiuseppe

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3087. Pages 127-144.

Abstract

Immunophenotyping by flow cytometry is an important component in the diagnostic evaluation of patients with acute lymphoblastic leukemia. This technique further permits the detection of minimal residual disease after therapy, a robust prognostic factor that may guide individualized treatment. We describe here laboratory methods for both the initial characterization of lymphoblasts at diagnosis and the detection of rare leukemic lymphoblasts after treatment. In addition to antibody combinations suitable for diagnosis and detection of minimal residual disease, we describe procedures for peripheral blood and bone marrow sample preparation, procedures for labeling of cell-surface and intracellular proteins with fluorochrome-conjugated antibodies, and approaches to analysis of immunophenotypic data, including those obtained in patients following CD19-targeted therapies.

PMID:
42814326
Bibliographic data and abstract were imported from PubMed on 01 Oct 2026.

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