Authors
Thi Thanh-Qui Nguyen, Daheui Choi, Seonhwa Lee, Jose M de Hoyos-Vega, P Vineeth Daniel, Amy S Mauer, Michael J Eller, Anthony Giron, Diana F Cedillo-Alcantar, Gulnaz Stybayeva, Rondell P Graham, Alina M Allen, Konstantinos N Lazaridis, Harmeet Malhi, Alexander Revzin
Published in
medRxiv : the preprint server for health sciences. Sep 07, 2026. Epub Sep 07, 2026.
Abstract
Accurate, noninvasive biomarkers for diagnosis and longitudinal monitoring of metabolic dysfunction-associated steatohepatitis (MASH) remain an urgent unmet clinical need. Here, we describe an electrochemical immunoassay that involves capturing hepatic extracellular vesicles (EVs) on antibody-modified electrodes followed by detecting EV-borne biomarkers using redox active gold nanoparticles (AuNPs). The intensity of electrochemical redox signal represents a quantitative measure of biomarker abundance. Three MASH-associated biomarkers-S100A11, TNF-α, and TGF-β1-were analyzed on hepatic EVs to assess steatotic, inflammatory, and fibrotic states of the diseased liver. The electrochemical signals for individual biomarkers were added into a composite EV MASH score. Plasma samples from 28 patients with MASH, 22 healthy controls, and 27 patients with non-MASH liver disease were scored in this manner, revealing 100% accuracy in the training cohort and >90% accuracy in an independent validation cohort. Our electrochemical immunosensor represents a minimally invasive liquid liver biopsy, offering a promising alternative to solid liver biopsies for screening and monitoring of MASH.
Circulating hepatic extracellular vesicles carry biomarkers of liver injury enabling accurate, minimally invasive MASH diagnosis in blood.
PMID:
42818423
Bibliographic data and abstract were imported from PubMed on 01 Oct 2026.
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