Authors
Tingyue Yang, Jiazhen Shi, Yinghua Song, Xiaojuan Niu, Yuangen Wu, Zhaoyu Wang
Published in
Mikrochimica acta. Volume 193. Issue 10. Oct 01, 2026. Epub Oct 01, 2026.
Abstract
Residues of tetracycline antibiotics (TCs) in food pose a serious threat to human health, creating an urgent need for the development of simple, rapid, and highly sensitive detection methods. Herein, guanosine monophosphate-iron coordination nanozyme (Fe-GMP) were synthesized using the self-assembly of guanosine-5'-monophosphate and iron. Based on the peroxidase-mimicking activity of Fe-GMP nanozyme, a novel colorimetric sensor for the colorimetric detection of TCs was developed. Fe-GMP catalyzes the oxidation of chromogenic substrate 3,3',5,5'-tetramethylbiphenylamine (TMB) in the presence of H2O2, producing a characteristic absorption peak at 652 nm. Interestingly, the introduction of TCs significantly enhances the peroxidase-like catalytic activity of Fe-GMP, causing the solution color to deepen from light to dark blue. Under optimized experimental conditions, the sensor exhibited a linear detection range of 0.2 µmol·L- 1 to 6 µmol·L- 1 for tetracycline, with a limit of detection (LOD) as low as 57.9 nmol·L- 1 (26.7 µg·kg- 1). The sensor has been successfully applied to determine tetracycline antibiotics in real milk and honey samples, with spiked recoveries ranging from 90% to 94%. The developed sensor exhibits a typical "signal-on" response mode, effectively avoiding the false-positive issues caused by nonspecific inhibition in traditional "signal-off" sensors. Furthermore, the sensor is cost-effective, highly efficient, and easy to operate, demonstrating great potential for rapid screening in food safety.
PMID:
42821144
Bibliographic data and abstract were imported from PubMed on 02 Oct 2026.
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