Ex Vivo Microtumor Testing to Predict Chemotherapy Responses in Patients with Bladder Cancer.

Authors

Mathijs P Scholtes, Esmee Koedoot, Lieke J Ceton, Timothy J P Sijsenaar, Marta G Montero, Caithlyn Z Roeland, Robert J van Soest, Joost Leijte, Hossein Roshani, Ruben Korthorst, Dieudonné J van der Meer, Geert J L H van Leenders, Willemijn Vader, Joost L Boormans, Tahlita C M Zuiverloon

Published in

European urology open science. Volume 93. Pages 3-11. Epub Sep 18, 2026.

Abstract

Patients with bladder cancer (BC) may receive intravesical or systemic chemotherapy, but predictive biomarkers to guide treatment selection are lacking. Consequently, some patients receive ineffective treatment, resulting in unnecessary toxicity and an increased risk of tumor progression.
To evaluate the feasibility of ex vivo micro-tumor testing for predicting chemotherapy response in patients with BC.
In this prospective study, 65 patients with BC were enrolled (Institutional Review Board no. MEC-2022-0233). Tumor samples were collected during transurethral resection or radical cystectomy. BC was confirmed by uropathological review, supported by the detection of BC-associated mutations in hTERT, FGFR3, and PIK3CA. Patient-derived micro-tumors were exposed ex vivo to anticancer drugs, and responses were assessed using image-based measurements of viability and morphology.
The primary outcome was successful completion of ex vivo drug testing within 14 days of tissue collection. The secondary outcome was concordance between ex vivo sensitivity and clinical response to platinum-based chemotherapy. Clinical response data were available for nine patients.
Ex vivo drug testing was successful for 48 of 65 patients (74%), with all successful results generated within 14 days of tissue collection. Among the nine patients with evaluable clinical outcomes following platinum-based chemotherapy, ex vivo responses corresponded with observed treatment responses. The main limitations were the small clinical correlation cohort and the limited number of drugs evaluated.
Ex vivo micro-tumor drug testing is feasible for BC within a clinically relevant timeframe. The observed correspondence between ex vivo and clinical responses to platinum-based chemotherapy supports further investigation of this approach for personalized treatment selection in BC.

PMID:
42820048
Bibliographic data and abstract were imported from PubMed on 02 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.