Authors
Hezheng Lyu, Hassan Gharibi, Bohdana Sokolova, Mücahit Varli, Anna Voiland, Brady Nilsson, Zhaowei Meng, Massimiliano Gaetani, Amir Ata Saei, Roman A Zubarev
Published in
Communications chemistry. Volume 9. Issue 1. Oct 02, 2026. Epub Oct 02, 2026.
Abstract
Knowledge of the targets of therapeutic compounds is vital for understanding their action mechanisms and side effects, but such valuable data is seldom available. The multiple complementary techniques needed for comprehensive target characterization must combine data reliability with sufficient analysis throughput. Here, we leveraged the Proteome Integral Solubility Alteration (PISA) assay to characterize the targets of 67 approved and experimental compounds against two main lung cancer subtypes, which is now provided through the website https://thermotargetminer.serve.scilifelab.se/app/thermotargetminer . Novel target candidates (pro-targets) were found for 77% of the tested molecules. Comparison of the protein solubility shifts in lysate vs. living cells highlighted the targets directly interacting with the compounds. We verified that the drug PEITC exerts cytotoxicity through the inhibition of a pro-target PAFAH1B. As PISA is now joining the arsenal of fast and reliable target characterization techniques, the presented database, ThermoTargetMiner, will become a useful resource in lung cancer research.
PMID:
42827103
Bibliographic data and abstract were imported from PubMed on 03 Oct 2026.
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