Authors
Khairil Anwar, Shota Kaneko, Itsuki Kawakita, Daffa Sean Adinegoro, Monami Kihara, Takaaki Kojima, Teruyo Ojima-Kato, Hideo Nakano
Published in
Journal of bioscience and bioengineering. Oct 03, 2026. Epub Oct 03, 2026.
Abstract
Antibodies play essential roles in mediating interactions between the host and microbiota. Despite their importance, the specificity and functional properties of naturally occurring antibodies reactive to gut microbiota remain poorly characterized, particularly at the monoclonal level. Here, we employed Ecobody technology, a single-B-cell-based antibody screening platform, to identify intestinal bacteria-reactive antigen-binding fragments (Fabs) from a non-immunized rabbit. Using Bacteroides cellulosilyticus as a model antigen, we identified and expressed Fabs using cell-free protein synthesis (CFPS) and subsequently produced a selected Fab in Brevibacillus choshinensis for detailed functional characterization. The purified Fab exhibited broad reactivity toward various intestinal bacteria, including both gram-positive and gram-negative bacteria. Conversely, it showed no binding to Saccharomyces cerevisiae or HEK293T cells, indicating specificity toward bacterial-associated antigens. Notably, the selected Fab demonstrated high binding affinity toward B. cellulosilyticus (KD = 2.41 nM). These results provide monoclonal-level evidence that antibodies reactive to intestinal bacteria are present in the peripheral B-cell repertoire of non-immunized hosts. Furthermore, this study demonstrates that the integration of Ecobody technology with the B. choshinensis expression system provides a feasible strategy for facilitating further investigation into host antibody-microbiota interactions.
PMID:
42829234
Bibliographic data and abstract were imported from PubMed on 04 Oct 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 7
- Comments 0