Double-crystallized GOx-entrapped copper nanoflowers enabling interfacial cascade catalysis for electrochemical glucose sensing.

Authors

Dain Kim, Jun Han Bae, Jangyong Kim, Kyung Hwa Kim, Ee Taek Hwang

Published in

Journal of materials chemistry. B. Oct 05, 2026. Epub Oct 05, 2026.

Abstract

Artificial enzyme-nanozyme cascade systems that enable interfacial coupling between biocatalytic and inorganic redox processes are of significant interest for biointerface engineering. In this study, a double-crystallized Cu nanoflower-based enzyme-nanozyme cascade platform was introduced by immobilizing GOx-integrated Cu nanoflowers (D-Cu@GOx NFs) onto an electrode surface. The double-crystallization process generates a hierarchical Cu nanoflower architecture that spatially confines enzyme and nanozyme components, enabling proximity-driven cascade catalysis through a redox-active Cu framework. Electrochemical characterization revealed that incorporation of GOx slightly modified the interfacial redox behavior while maintaining quasi-reversible electron-transfer characteristics. Chronoamperometric measurements demonstrated concentration-dependent responses toward both H₂O₂ and glucose, confirming that the Cu nanoflower structure retains intrinsic peroxidase-like activity through reversible Cu²⁺/Cu⁺ redox cycling. The D-Cu@GOx NF modified electrode exhibited a linear glucose response up to 31200 µM, with analytical parameters calculated from the linear region below 5200 µM. These results indicate that the integrated D-Cu@GOx NF architecture retains both enzymatic glucose-responsive and Cu-associated electrochemical functionality, supporting its application as a hybrid enzyme-nanozyme electrochemical interface. This work demonstrates a double-crystallized nanoflower platform as a biointerface for integrating enzyme-nanozyme cascade systems.

PMID:
42831254
Bibliographic data and abstract were imported from PubMed on 05 Oct 2026.

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