Authors
Hiroaki Kato, Tomohiro Fuse, Shoko Sato, Yohei Kurihara, Wataru Kagawa, Takeshi Urano, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Mitsuhiro Shimizu
Published in
Genome research. Volume 36. Issue 7. Pages 1417-1428. Jul 07, 2026. Epub Jul 07, 2026.
Abstract
The nucleosome is a structural and functional subunit of chromatin, and its positioning in eukaryotic genomes serves as a key platform for gene regulation. Here, we determine the positions of fully wrapped nucleosomes across the yeast genome by chemical mapping through the histone H2A-A122C residue, which cleaves near the DNA entry/exit sites. This approach reveals the most refined sequence-dependent profile of nucleosomes reported to date. Comparisons of the H2A-A122C approach with the H3-Q85C and H4-S47C methods clearly show a sequence preference for the chemical cleavage site. Notably, depletion of CC and GG dinucleotides at nucleotide positions -11 to -9 and +9 to +11 bp, respectively, from the nucleosome dyad position (zero) is consistently accompanied by enrichment of AA/AT/TA/TT dinucleotides in both yeast and mouse genomes. Introducing consecutive C•G base pairs to the corresponding sites in the Widom 601 sequence makes reconstituted nucleosomes more likely to shift into alternative positions without affecting the thermal stability of the nucleosome particle, implying a structural constraint imposed by the DNA sequence. Thus, CC and GG dinucleotides in the major groove blocks, in which the minor groove faces out from the histone octamer, at superhelix locations (SHL) -1.0 and +1.0, respectively, destabilize histone-DNA interactions, serving as intrinsic determinants of nucleosome positioning in eukaryotic genomes.
PMID:
42833892
Bibliographic data and abstract were imported from PubMed on 06 Oct 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 10
- Comments 0