Authors
Ruibao Ju, Xin Wang, Ju Peng, Lixin Xia, Zhiwen Jiang, Jinhua Chi, Shuo Wang, Baoqin Han
Published in
International journal of biological macromolecules. Pages 154740. Oct 05, 2026. Epub Oct 05, 2026.
Abstract
To develop high-efficiency materials for skin photodamage repair, in this study, carboxymethyl chitosan-pyroglutamic acid (CMCS-Pyr) was synthesized by grafting pyroglutamic acid onto carboxymethyl chitosan (CMCS) via an amide reaction， achieving a grafting rate of 19.28%. Rheological analysis identified 10% as the optimal application concentration of CMCS-Pyr. In vitro experiments demonstrated that the material had a hemolysis rate of less than 5% and no skin irritation. It exhibits multiple free radical scavenging capacities, with ABTS, DPPH and ·OH radical scavenging rates of 52.8%, 50.9% and 90.5%, respectively. CMCS-Pyr could significantly enhance the superoxide dismutase (SOD) activity of HaCaT cells, and promote cell migration as well as collagen synthesis. In animal experiments based on the mouse UVB-induced skin photodamage model, CMCS-Pyr remarkably increased the water content and maximum tensile strength of damaged skin, inhibited the expressions of matrix metalloproteinase-9 (MMP-9) and nuclear factor-kappa B p65 (NF-κB-p65), and reduced the malondialdehyde (MDA) content. Transcriptome and quantitative real-time polymerase chain reaction (qPCR) analyses confirmed that CMCS-Pyr up-regulated the expressions of genes such as COL1 and COL3 and down-regulated inflammatory factors by regulating the MAPK/NF-κB signaling pathway, thereby exerting a synergistic effect of anti-inflammation, anti-oxidation and pro-repair. Collectively, CMCS-Pyr is a promising material for skin photodamage repair.
PMID:
42833328
Bibliographic data and abstract were imported from PubMed on 06 Oct 2026.
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