Authors
Ghazaleh Eshaghi, Hamid Reza Rasouli, David Kaiser, Tobias Fischer, Katrin Frankenfeld, Abhishek Sharma, Andrey Turchanin
Published in
Advanced healthcare materials. Pages e71776. Oct 05, 2026. Epub Oct 05, 2026.
Abstract
The simultaneous circulation of multiple respiratory viruses demands diagnostic platforms capable of rapid, sensitive, and multiplexed detection in complex clinical samples. Here, we present a label-free, multi-channel surface plasmon resonance (MC-SPR) biosensor for simultaneous, real-time detection of three major respiratory viruses. A molecular 2D material, ∼1 nm thick azide-terminated carbon nanomembrane (N3-CNM), is used to functionalize the MC-SPR sensor surface, enabling covalent antibody immobilization via click chemistry. This approach provides controlled functionalization of multiple channels with virus-specific antibodies, resulting in robust and reproducible multiplexed detection of the respective antigens. We demonstrate specific detection of SARS-CoV-2, Influenza A, and respiratory syncytial virus (RSV) antigens from their mixtures, both in physiological buffer and clinically relevant nasopharyngeal swab matrices, with sensitivity in the pM range, negligible cross-reactivity, and high reproducibility. Unknown analyte concentrations can be directly determined from the SPR data with high accuracy using the respective calibration curves. This work establishes a robust and versatile platform based on molecular 2D materials for the development of multiplexed biosensors and medical diagnostic applications.
PMID:
42834624
Bibliographic data and abstract were imported from PubMed on 06 Oct 2026.
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