Authors
Tingting Lai, Yi Lin, Fuping Ding, Zhenni Shi, Jun Zhang, Yongjin Yan, Shunzhong Gu, Kunting Zhong, Jinhong Liu, Weiyu Liang, Yilin Duan, Zhanhui Huang, Shuhe Zhang, Jin Zhang
Published in
Adipocyte. Volume 15. Issue 1. Pages 2719272. Epub Oct 06, 2026.
Abstract
Finding bioactive substances that can promote the de novo differentiation of stem cells into thermogenic adipocytes is a promising anti-obesity strategy. Cinnamaldehyde (CM) and 18β-glycyrrhetinic acid (GA) are the main active ingredients in Chinese herbal medicine. In this study, C3H10T1/2 mesenchymal stem cells (MSCs) were used to investigate whether CM and GA could promote the differentiation of MSCs into thermogenic adipocytes and the possible molecular mechanism. The experimental results demonstrated that CM and GA could promote the mRNA expression of adipogenic transcription factors Pparγ, Cebpα and Cebpβ, as well as thermogenic adipocyte-specific markers Pgc1α and Ucp1. CM combined with GA further enhanced these effects. CM/GA alone or in combination could increase the expression of FNDC5 mRNA and protein. After transfection with FNDC5 siRNA, the mRNA and protein levels of FNDC5 and UCP1 were significantly decreased, while the levels of PGC1α did not change apparently. After the combination of CM and GA intervention, the levels of FNDC5 and UCP1 were slightly restored, but could not return to the normal level. In conclusion, CM and GA could promote the differentiation of C3H10T1/2 MSCs into thermogenic adipocytes, and their combination could enhance this effect, which may be mediated by PGC1α/FNDC5/UCP1 signalling pathway.
PMID:
42836659
Bibliographic data and abstract were imported from PubMed on 06 Oct 2026.
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