Protocol for analyzing tumor-infiltrating bystander CD8⁺ T cells in mice using adoptive transfer of P14 TCR-transgenic CD8⁺ T cells.

Authors

Chunyang Xie, Wenwen Xi, Hairu Wang, Lilin Ye, Xiangyu Chen, Yao Lin

Published in

STAR protocols. Volume 7. Issue 4. Pages 104886. Oct 06, 2026. Epub Oct 06, 2026.

Abstract

While tumor-specific CD8⁺ T cells become functionally exhausted within the tumor microenvironment (TME), virus-specific bystander memory CD8⁺ T (T_BYS) cells are abundant and represent valuable immunotherapy targets. Here, we present a protocol to analyze tumor-infiltrating virus-specific T_BYS cells in murine tumor models using P14 CD8⁺ T cells. We describe steps for monitoring long-term in vivo behavior of P14 T_BYS cells and characterizing their differentiation trajectories and functional heterogeneity. For complete details on the use and execution of this protocol, please refer to Lin et al.¹.

PMID:
42837264
Bibliographic data and abstract were imported from PubMed on 07 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.