Tailored speckle illumination microscopy with enhanced sectioning and image quality.

Authors

SeungYun Han, KyeoReh Lee, Young Seo Kim, Chuan Li, Nicholas Bender, Kabish Wisal, Taeyun Ku, Jerome Mertz, Hui Cao

Published in

Proceedings of the National Academy of Sciences of the United States of America. Volume 123. Issue 41. Pages e2617763123. Oct 13, 2026. Epub Oct 06, 2026.

Abstract

Optical speckle patterns have been widely used for illumination in computational imaging, optical sectioning microscopy, and superresolution imaging. However, commonly used speckles satisfy Rayleigh statistics, which are not ideal for diverse imaging applications. Here we tailor three-dimensional speckle intensity statistics for dynamic speckle illumination microscopy based on linear fluorescence. Optical sectioning is enhanced by axially varying speckle contrast, and image reconstruction noise is minimized with in-focus speckles of binary intensities. The customized speckle statistics are shown to tolerate sample-induced aberration and scattering. We apply tailored speckle illumination to mouse brain vascular imaging and demonstrate much improved image quality than optical-sectioning structured illumination. These results establish customization of speckle intensity statistics as a promising strategy for robust, high-throughput fluorescence imaging in thick, scattering biological specimens.

PMID:
42837451
Bibliographic data and abstract were imported from PubMed on 07 Oct 2026.

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